ARG43158
anti-RNF125 antibody
anti-RNF125 antibody for Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RNF125 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RNF125 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-105 of Human RNF125 (NP_060301.2). |
| Conjugation | Un-conjugated |
| Alternate Names | RING finger protein 125; EC 6.3.2.-; T-cell RING activation protein 1; E3 ubiquitin-protein ligase RNF125; TRAC1; TRAC-1; TNORS |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | K562 | ||||
| Observed Size | ~ 28 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96EQ8 Human E3 ubiquitin-protein ligase RNF125 Swiss-port # Q9D9R0 Mouse E3 ubiquitin-protein ligase RNF125 |
|---|---|
| Gene Symbol | RNF125 |
| Gene Full Name | ring finger protein 125, E3 ubiquitin protein ligase |
| Background | This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway. [provided by RefSeq, Mar 2012] |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as DDX58/RIG-I, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53 (PubMed:15843525, PubMed:17460044, PubMed:17643463, PubMed:26027934, PubMed:26471729, PubMed:25591766, PubMed:27411375). Acts as a negative regulator of type I interferon production by mediating ubiquitination of DDX58/RIG-I at 'Lys-181', leading to DDX58/RIG-I degradation (PubMed:17460044, PubMed:26471729). Mediates ubiquitination and subsequent degradation of p53/TP53 (PubMed:25591766). Mediates ubiquitination and subsequent degradation of JAK1 (PubMed:26027934). Acts as a positive regulator of T-cell activation (PubMed:15843525). [UniProt] |
| Cellular Localization | Golgi apparatus membrane; Lipid-anchor. Note=Shows a reticular staining pattern within the cell and is probably expressed at other intracellular membranes in addition to the Golgi membrane. Not detected at the plasma membrane. [UniProt] |
| Calculated MW | 26 kDa |
| PTM | Autoubiquitinated, leading to its subsequent proteasomal degradation. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG43158 anti-RNF125 antibody WB image
Western blot: 25 µg of K562 cell lysate stained with ARG43158 anti-RNF125 antibody at 1:1000 dilution.
