ARG59737
anti-RPL14 antibody
anti-RPL14 antibody for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RPL14 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RPL14 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 173-204 of Human RPL14. |
| Conjugation | Un-conjugated |
| Alternate Names | 60S ribosomal protein L14; L14; RL14; CTG-B33; hRL14; CAG-ISL 7; CAG-ISL-7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | MCF7 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RPL14 |
| Gene Full Name | ribosomal protein L14 |
| Background | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| Calculated MW | 23 kDa |
Images (3) Click the Picture to Zoom In
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ARG59737 anti-RPL14 antibody ICC/IF image
Immunofluorescence: 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells stained with ARG59737 anti-RPL14 antibody (green) at 1:25 dilution. The nuclear counter stain is DAPI (blue).
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ARG59737 anti-RPL14 antibody WB image
Western blot: 20 µg of MCF7 cell lysate stained with ARG59737 anti-RPL14 antibody at 1:2000 dilution.
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ARG59737 anti-RPL14 antibody FACS image
Flow Cytometry: U-2OS cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. Cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG59737 anti-RPL14 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
