ARG40701
anti-RPL19 antibody
anti-RPL19 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RPL19 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Bov, Chk, Dog, Mk, Rb |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RPL19 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 132-170 of Human RPL19. (FKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEAR) |
| Conjugation | Un-conjugated |
| Alternate Names | 60S ribosomal protein L19; L19 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RPL19 |
| Gene Full Name | ribosomal protein L19 |
| Background | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L19E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| Calculated MW | 23 kDa |
| PTM | Citrullinated by PADI4. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG40701 anti-RPL19 antibody ICC/IF image
Immunofluorescence: U20S cells stained with ARG40701 anti-RPL19 antibody at 2 µg/ml, overnight at 4°C. Cells was counterstained with DAPI (blue).
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ARG40701 anti-RPL19 antibody FACS image
Flow Cytometry: U20S cells were blocked with 10% normal goat serum and stained with ARG40701 anti-RPL19 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG40701 anti-RPL19 antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and stained with ARG40701 anti-RPL19 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG40701 anti-RPL19 antibody WB image
Western blot: Rat liver, HEPA and HepG2 whole cell lysates stained with ARG40701 anti-RPL19 antibody at 0.5 µg/ml.
