ARG45802

anti-RPN2 antibody

anti-RPN2 antibody for Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes RPN2
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RPN2
Antigen Species Human
Immunogen Recombinant protein containing to human RPN2.
Conjugation Un-conjugated
Alternate Names RPN2; RPNII; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; RPN-II; RIBIIR; EC 2.4.99.18; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; Ribophorin-2; SWP1; Ribophorin II

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 69 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6185 Human RPN2

Swiss-port # P04844 Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

Gene Symbol RPN2
Gene Full Name ribophorin II
Background This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Function Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. [UniProt]
Cellular Localization Endoplasmic reticulum. Endoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt]
Calculated MW 69 kDa
PTM Glycoprotein; Isopeptide bond; Ubl conjugation. [UniProt]