ARG55465
anti-RRM1 antibody
anti-RRM1 antibody for ICC/IF,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RRM1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RRM1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human RRM1 (NP_001024.1) |
| Conjugation | Un-conjugated |
| Alternate Names | Ribonucleoside-diphosphate reductase large subunit; R1; EC 1.17.4.1; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit; RIR1; RR1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07742 Mouse Ribonucleoside-diphosphate reductase large subunit Swiss-port # P23921 Human Ribonucleoside-diphosphate reductase large subunit |
|---|---|
| Gene Symbol | RRM1 |
| Gene Full Name | ribonucleotide reductase M1 |
| Background | This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq, Jul 2008] |
| Function | Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 90 kDa |
Images (2) Click the Picture to Zoom In
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ARG55465 anti-RRM1 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55465 anti-RRM1 antibody at 1:50 dilution. Left: primary antibody (red). Right: DAPI (blue).
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
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ARG55465 anti-RRM1 antibody WB image
Western blot: 30 µg of HeLa and SW480 cell lysates stained with ARG55465 anti-RRM1 antibody at 1:500 dilution.
Customer's Feedback
Excellent
Review for anti-RRM1 antibody
Application：WB
Sample：293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-RRM1 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:50
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
Review for anti-RRM1 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:50
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
