ARG66766
anti-RRM1 antibody [SQab20199]
anti-RRM1 antibody [SQab20199] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab20199] recognizes RRM1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab20199 |
| Isotype | IgG |
| Target Name | RRM1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 700-800 of Human RRM1. |
| Conjugation | Un-conjugated |
| Alternate Names | Ribonucleoside-diphosphate reductase large subunit; R1; EC 1.17.4.1; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit; RIR1; RR1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). WB: for mouse and rat samples, maybe more antibody is needed, and mouse and rat samples were tested only by WB. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Breast carcinoma tissue. | ||||||
| Observed Size | 90 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07742 Mouse Ribonucleoside-diphosphate reductase large subunit Swiss-port # P23921 Human Ribonucleoside-diphosphate reductase large subunit |
|---|---|
| Gene Symbol | RRM1 |
| Gene Full Name | ribonucleotide reductase M1 |
| Background | This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
| Function | Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 90 kDa |
Images (2) Click the Picture to Zoom In
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ARG66766 anti-RRM1 antibody [SQab20199] WB image (Customer's Feedback)
Western blot: MCF7 cell lysate, HeLa cell lysate, Mouse heart lysate and Rat heart lysate stained with ARG66766 anti-RRM1 antibody [SQab20199] at 1:1000 dilution.
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ARG66766 anti-RRM1 antibody [SQab20199] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human breast carcinoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66766 anti-RRM1 antibody [SQab20199] at 18°C - 25°C for 30 minutes.
Customer's Feedback
Excellent
Review for anti-RRM1 antibody [SQab20199]
Application：WB
Sample：MCF7, HeLa, Mouse heart, Rat heart
Sample Loading Amount：20 μg
SDS Page Type：Denatured
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
