ARG41230
anti-RUFY1 antibody
anti-RUFY1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes RUFY1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RUFY1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human RUFY1. (C-QKNEAITSFEGKT) (NP_079434.3; NP_001035541.1) |
| Conjugation | Un-conjugated |
| Alternate Names | FYVE-finger protein EIP1; RUN and FYVE domain-containing protein 1; ZFYVE12; Zinc finger FYVE domain-containing protein 12; Rab4-interacting protein; La-binding protein 1; RABIP4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96T51 Human RUN and FYVE domain-containing protein 1 |
|---|---|
| Gene Symbol | RUFY1 |
| Gene Full Name | RUN and FYVE domain containing 1 |
| Background | This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
| Function | Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking. [UniProt] |
| Cellular Localization | Cytoplasm. Early endosome membrane; Peripheral membrane protein. [UniProt] |
| Calculated MW | 80 kDa |
| PTM | Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG41230 anti-RUFY1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue stained with ARG41230 anti-RUFY1 antibody at 5 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
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ARG41230 anti-RUFY1 antibody WB image
Western blot: 35 µg of HeLa cell lysate (in RIPA buffer) stained with ARG41230 anti-RUFY1 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.
