ARG45298
anti-RanBP2 antibody
anti-RanBP2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Polyclonal antibody recognizes RanBP2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RanBP2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human RanBP2. |
| Conjugation | Un-conjugated |
| Alternate Names | RANBP2; RAN Binding Protein 2; NUP358; ADANE; Acute Necrotizing Encephalopathy 1 (Autosomal Dominant); Nuclear Pore Complex Protein Nup358; E3 SUMO-Protein Ligase RanBP2; Ran-Binding Protein 2; 358 KDa Nucleoporin; Nucleoporin Nup358; Nucleoporin 358; ANE1; P270; E3 SUMO-Protein Transferase RanBP2; Transformation-Related Protein 2; EC 2.3.2.-; EC 5.2.1.8 47; RanBP2; IIAE3; TRP1; TRP2== |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 358 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RANBP2 |
| Gene Full Name | RAN Binding Protein 2 |
| Background | RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. The encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q. [provided by RefSeq, Jul 2008] |
| Function | E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. [UniProt] |
| Cellular Localization | Membrane; Nuclear pore complex; Nucleus. [UniProt] |
| Calculated MW | 358 kDa |
| PTM | Acetylation; Disulfide bond; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation. [UniProt] |
