ARG52421
anti-SAP49 antibody [3A1]
anti-SAP49 antibody [3A1] for ICC/IF,Western blot and Human,Mouse,Rat,Bovine
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [3A1] recognizes SAP49 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3A1 |
| Isotype | IgG2b |
| Target Name | SAP49 |
| Antigen Species | Human |
| Immunogen | Recombinant full length human SAP49 expressed in and purified from E. Coli. |
| Conjugation | Un-conjugated |
| Alternate Names | SAP 49; SF3b50; SAP49; Hsh49; AFD1; Splicing factor 3B subunit 4; SF3b49; Pre-mRNA-splicing factor SF3b 49 kDa subunit; Spliceosome-associated protein 49 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | Specific for the ~49k SAP49 protein * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | PBS and 10 mM Sodium azide |
| Preservative | 10 mM Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SF3B4 |
| Gene Full Name | splicing factor 3b, subunit 4, 49kDa |
| Background | The spliceosome associated protein of 49 kDa (SAP49), also known as SF3B4, is a widely expressed nuclear splicing factor that has been shown to have RNA binding activity as well as direct interaction with SAP145 (Champion-Arnaud and Reed 1994). The SAP145-SAP49 complex has recently been implicated in cell cycle progression as Vpr (viral protein 1 of HIV-1) has been shown to bind SAP145 thus interfering with the proper formation and functioning of the SAP145-SAP49 complex (Terada and Yasuda 2006). |
| Research Area | Gene Regulation antibody |
| Calculated MW | 44 kDa |
Images (3) Click the Picture to Zoom In
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ARG52421 anti-SAP49 antibody [3A1] WB image
Western blot: HeLa lysate stained with ARG52421 anti-SAP49 antibody [3A1] showing specific immunolabeling of the ~ 49k SAP49 protein.
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ARG52421 anti-SAP49 antibody [3A1] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG52421 anti-SAP49 antibody [3A1] showing strong granular nuclear staining of SAP49 in red and vimentin (ARG52468 anti-vimentin antibody) in green.
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ARG52421 anti-SAP49 antibody [3A1] WB image
Western blot: NIH/3T3 cytosol, NIH/3T3 nuclear, HeLa cytosol and HeLa nuclear fractions stained with ARG52421 anti-SAP49 antibody [3A1] (green) at 1:1000 dilution.
The same blot was simultaneously stained with anti-GAPDH antibody (red) at 1:20000 dilution.
