ARG65697
anti-SIRT1 antibody
anti-SIRT1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SIRT1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SIRT1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide around the C-terminus of Human SIRT1. |
| Conjugation | Un-conjugated |
| Alternate Names | 75SirT1; SIR2L1; SIR2alpha; SIR2-like protein 1; EC 3.5.1.-; NAD-dependent protein deacetylase sirtuin-1; SIR2; hSIRT1; Regulatory protein SIR2 homolog 1; hSIR2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Hela, F9, Jurkat, human lung carcinoma tissue, human colon carcinoma tissue |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 1*TBS (pH 7.4), 0.05% Sodium azide, 0.5% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.5% BSA, 40% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q923E4 Mouse NAD-dependent protein deacetylase sirtuin-1 Swiss-port # Q96EB6 Human NAD-dependent protein deacetylase sirtuin-1 |
|---|---|
| Gene Symbol | SIRT1 |
| Gene Full Name | sirtuin 1 |
| Background | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody |
| Calculated MW | 82 kDa |
| PTM | Methylated on multiple lysine residues; methylation is enhanced after DNA damage and is dispensable for deacetylase activity toward p53/TP53. Phosphorylated. Phosphorylated by STK4/MST1, resulting in inhibition of SIRT1-mediated p53/TP53 deacetylation. Phosphorylation by MAPK8/JNK1 at Ser-27, Ser-47, and Thr-530 leads to increased nuclear localization and enzymatic activity. Phosphorylation at Thr-530 by DYRK1A and DYRK3 activates deacetylase activity and promotes cell survival. Phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) at Ser-47 inhibits deacetylation activity. Phosphorylated by CaMK2, leading to increased p53/TP53 and NF-kappa-B p65/RELA deacetylation activity (By similarity). Phosphorylation at Ser-27 implicating MAPK9 is linked to protein stability. There is some ambiguity for some phosphosites: Ser-159/Ser-162 and Thr-544/Ser-545. Proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield catalytic inactive but stable SirtT1 75 kDa fragment (75SirT1). S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity. |
Images (2) Click the Picture to Zoom In
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ARG65697 anti-SIRT1 antibody WB image
Western blot: 30 µg of HCT116 cell lysate stained with ARG65697 anti-SIRT1 antibody at 1:1000 dilution.
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ARG65697 anti-SIRT1 antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG65697 anti-SIRT1 antibody at 1:1000 dilution.
Customer's Feedback
Excellent
Review for anti-SIRT1 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
