ARG63095
anti-SIT antibody [SIT-01]
anti-SIT antibody [SIT-01] for Flow cytometry,Immunoprecipitation,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [SIT-01] recognizes SIT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IP, WB |
| Specificity | The clone SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SIT-01 |
| Isotype | IgG1 |
| Target Name | SIT |
| Antigen Species | Human |
| Immunogen | Bacterially produced recombinant intracellular fragment of human SIT. |
| Conjugation | Un-conjugated |
| Alternate Names | gp30/40; Signaling threshold-regulating transmembrane adapter 1; SIT; SHP2-interacting transmembrane adapter protein; SIT-R; Suppression-inducing transmembrane adapter 1 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Staining technique: Intracellular staining. WB: WB analysis demonstrated that SIT is expressed as an ~ 40 kDa protein that is reduced to ~ 20 kDa by endoglycosidase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y3P8 Human Signaling threshold-regulating transmembrane adapter 1 |
|---|---|
| Gene Symbol | SIT1 |
| Gene Full Name | signaling threshold regulating transmembrane adaptor 1 |
| Background | SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors. |
| Function | Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 21 kDa |
| PTM | Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK. |
Images (1) Click the Picture to Zoom In
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ARG63095 anti-SIT antibody [SIT-01] WB image
Western blot: Jurkat cell lysate (1% lauryl maltoside) (non-reduced sample) stained with ARG63095 anti-SIT antibody [SIT-01].
