ARG22658
anti-SLA Class II DR antibody [2E9/13]
anti-SLA Class II DR antibody [2E9/13] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Bovine,Pig
Overview
| Product Description | Mouse Monoclonal antibody [2E9/13] recognizes SLA Class II DR This antibody recognizes SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognizes a monomorphic determinant of Porcine SLA DR. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR. Mouse anti Pig SLA class II Dr, clone 2E9/13 immunoprecipitates a heterodimer composed of two polypeptides of 28 and 35kD from NP-40 extracts of biotin surface-labelled Porcine peripheral blood mononuclear cells. Clone 2E9/13 is reported to inhibit the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B (Bullido et al. 1997). |
|---|---|
| Tested Reactivity | Bov, Pig |
| Tested Application | FACS, IHC-Fr, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2E9/13 |
| Isotype | IgG2b |
| Target Name | SLA Class II DR |
| Antigen Species | Pig |
| Immunogen | Porcine monocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | SLA-DRA1; SLA-DRA2; SLA-DR-alpha |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | SLA-DRA |
|---|---|
| Gene Full Name | MHC class II DR-alpha |
Images (1) Click the Picture to Zoom In
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ARG22658 anti-SLA Class II DR antibody [2E9/13] FACS image
Flow Cytometry: Pig peripheral blood lymphocytes stained with ARG22658 anti-SLA Class II DR antibody [2E9/13] followed by Goat anti-Mouse IgG (FITC).
