ARG65201
anti-SLC12A2 antibody
anti-SLC12A2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SLC12A2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | FACS, ICC/IF, IHC-P |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SLC12A2 |
| Antigen Species | Human |
| Immunogen | C-NALQHSIRLSGVEDH |
| Conjugation | Un-conjugated |
| Alternate Names | BSC2; Basolateral Na-K-Cl symporter; Solute carrier family 12 member 2; potassium; PPP1R141; Bumetanide-sensitive sodium-; NKCC1; BSC |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 131 kDa |
Images (3) Click the Picture to Zoom In
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ARG65201 anti-SLC12A2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG65201 anti-SLC12A2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG65201 anti-SLC12A2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Colon. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65201 anti-SLC12A2 antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG65201 anti-SLC12A2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG65201 anti-SLC12A2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
