ARG41011
anti-SMARCA5 / SNF2H antibody
anti-SMARCA5 / SNF2H antibody for Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SMARCA5 / SNF2H |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SMARCA5 / SNF2H |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 100-400 of Human SMARCA5 (NP_003592.3). |
| Conjugation | Un-conjugated |
| Alternate Names | ISWI; Sucrose nonfermenting protein 2 homolog; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; hISWI; EC 3.6.4.-; SNF2H; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; hSNF2H; WCRF135 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | 293T | ||||
| Observed Size | 110 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60264 Human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin s Swiss-port # Q91ZW3 Mouse SWI/SNF-related matrix-associated actin-dependent regulator of chromatin s |
|---|---|
| Gene Symbol | SMARCA5 |
| Gene Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 |
| Background | The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008] |
| Function | Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 122 kDa |
Images (1) Click the Picture to Zoom In
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ARG41011 anti-SMARCA5 / SNF2H antibody WB image
Western blot: 25 µg of 293T cell lysate stained with ARG41011 anti-SMARCA5 / SNF2H antibody at 1:1000 dilution.
