ARG22295
anti-SOD2 antibody
anti-SOD2 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Chicken,D. melanogaster ,Dog,Guinea pig,Hamster,Invertebrates,Monkey,Pig,Rabbit,Sheep,Xenopus laevis
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SOD2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Chk, Dm, Dog, Gpig, Hm, Invt, Mk, Pig, Rb, Sheep, Xenopus laevis |
| Tested Application | ELISA, ICC/IF, IHC-P, IP, WB |
| Specificity | Detects ~25kDa. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | SOD2 |
| Antigen Species | Rat |
| Immunogen | Rat SOD2 |
| Conjugation | Un-conjugated |
| Alternate Names | MNSOD; Superoxide dismutase [Mn], mitochondrial; IPOB; EC 1.15.1.1; MVCD6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Sod2 |
| Gene Full Name | superoxide dismutase 2, mitochondrial |
| Background | This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. [UniProt] |
| Cellular Localization | Mitochondrion, Mitochondrion Matrix |
| Calculated MW | 25 kDa |
| PTM | Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting (By similarity). |
Images (4) Click the Picture to Zoom In
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ARG22295 anti-Mn-SOD antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed HeLa cells stained with ARG22295 anti-Mn-SOD antibody (yellow) at 1:120 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
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ARG22295 anti-Mn-SOD antibody IHC image
Immunohistochemistry: Bouin solution fixed Mouse backskin stained with ARG22295 anti-Mn-SOD antibody (green) at 1:100 dilution (1 hour).
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ARG22295 anti-Mn-SOD antibody WB image
Western blot: Rat Tissue lysates stained with ARG22295 anti-Mn-SOD antibody at 1:1000 dilution.
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ARG22295 anti-Mn-SOD antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed HeLa cells stained with ARG22295 anti-Mn-SOD antibody (red) at 1:120 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
