ARG45224
anti-SPT5 antibody
anti-SPT5 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SPT5 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SPT5 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human SUPT5H. |
| Conjugation | Un-conjugated |
| Alternate Names | SUPT5H; SPT5 Homolog, DSIF Elongation Factor Subunit; SPT5H; SPT5; DRB Sensitivity-Inducing Factor 160 KDa Subunit; DRB Sensitivity-Inducing Factor Large Subunit; Transcription Elongation Factor SPT5; Tat-Cotransactivator 1 Protein; DSIF Large Subunit; DSIF P160; FLJ34157; HSPT5; Suppressor Of Ty 5 Homolog (S. Cerevisiae); Suppressor Of Ty (S.Cerevisiae) 5 Homolog; Suppressor Of Ty 5 Homolog; Tat-CT1 Protein; Tat-CT1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 150 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00267 Human Transcription elongation factor SPT5 |
|---|---|
| Gene Symbol | SUPT5H |
| Gene Full Name | SPT5 Homolog, DSIF Elongation Factor Subunit |
| Background | Enables enzyme binding activity and protein heterodimerization activity. Involved in positive regulation of macroautophagy; regulation of DNA-templated transcription; and transcription elongation by RNA polymerase II. Located in nucleoplasm. Part of DSIF complex. [provided by Alliance of Genome Resources, Nov 2024] |
| Function | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 121 kDa |
| PTM | Acetylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation. [UniProt] |
