ARG54298
anti-STRO1 antibody [STRO-1]
anti-STRO1 antibody [STRO-1] for Flow cytometry,ICC/IF and Human
Controls and Markers antibody
Overview
| Product Description | Mouse Monoclonal antibody [STRO-1] recognizes STRO1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF |
| Specificity | The clone STRO-1 recognizes the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | STRO-1 |
| Isotype | IgM |
| Target Name | STRO1 |
| Antigen Species | Human |
| Immunogen | Human CD34 positive bone marrow cells |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by precipitation methods and ion exchange chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells. |
|---|---|
| Research Area | Controls and Markers antibody |
Images (1) Click the Picture to Zoom In
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ARG54298 anti-STRO1 antibody [STRO-1] FACS image
Flow Cytometry: Kg1a cells stained with ARG54298 anti-STRO1 antibody [STRO-1].
