ARG51794
anti-Smad 1 phospho (Ser465) antibody
anti-Smad 1 phospho (Ser465) antibody for Western blot and Human,Mouse,Rat
Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Smad 1 phospho (Ser465) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Smad 1 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 465 (I-S-S-V-S(p)) derived from Human Smad1. |
| Conjugation | Un-conjugated |
| Alternate Names | MADR1; Mad-related protein 1; Transforming growth factor-beta-signaling protein 1; Mothers against decapentaplegic homolog 1; MADH1; SMAD 1; Smad1; JV4-1; JV41; BSP-1; hSMAD1; BSP1; Mothers against DPP homolog 1; MAD homolog 1; SMAD family member 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P70340 Mouse Mothers against decapentaplegic homolog 1 Swiss-port # Q15797 Human Mothers against decapentaplegic homolog 1 |
|---|---|
| Gene Symbol | SMAD1 |
| Gene Full Name | SMAD family member 1 |
| Background | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30155 Phospho Smad1 for Activation / Inhibition site Antibody Panel Related products: Smad 1 antibodies; Smad 1 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 52 kDa |
| PTM | Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4. Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm (By similarity). |
Images (1) Click the Picture to Zoom In
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ARG51794 anti-Smad 1 phospho (Ser465) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with PMA stained with ARG51794 anti-Smad 1 phospho (Ser465) antibody.
