ARG22638
anti-Sulfotyrosine antibody [7C5]
anti-Sulfotyrosine antibody [7C5] for ICC/IF,Western blot and Other
Overview
| Product Description | Mouse Monoclonal antibody [7C5] recognizes Sulfotyrosine |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ICC/IF, WB |
| Specificity | Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7C5 |
| Isotype | IgG1 |
| Target Name | Sulfotyrosine |
| Antigen Species | Others |
| Immunogen | Synthetic Sulfotyrosine conjugated to KLH. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol. |
| Preservative | 0.9% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (2) Click the Picture to Zoom In
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ARG22638 anti-Sulfotyrosine antibody [7C5] ICC/IF image
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22638 anti-Sulfotyrosine antibody [7C5] at 1:50 for 30 - 60 min at RT. Secondary Antibody: Goat anti-Mouse (Alexa Fluor 488) at 1:1500 for 30 - 60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Primary antibody (D) Composite. .
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ARG22638 anti-Sulfotyrosine antibody [7C5] WB image
Western blot: 2.0 µg of Sulfotyrosine-BSA, Tyrosine-BSA, Phosphotyrosine-BSA, and BSA stained with ARG22638 anti-Sulfotyrosine antibody [7C5] at 1:1000 for 2 hours at RT.
