ARG52434
anti-Synaptojanin 1 antibody [5H1]
anti-Synaptojanin 1 antibody [5H1] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat
Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [5H1] recognizes Synaptojanin 1 |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5H1 |
| Isotype | IgG1 |
| Target Name | Synaptojanin 1 |
| Antigen Species | Rat |
| Immunogen | Recombinant protein from the C-terminal region of rat synaptojanin 1. |
| Conjugation | Un-conjugated |
| Alternate Names | PARK20; Synaptojanin-1; EC 3.1.3.36; Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1; INPP5G |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~145k synaptojanin I protein in Western blots of Rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to geneRate the antibody. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SYNJ1 |
| Gene Full Name | synaptojanin 1 |
| Background | Synaptojanin is a phosphatidylinositol phosphatase involved in clathrinmediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms; one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues (Ramjaun AR & McPherson PS, 1996). The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down’s syndrome (DS). It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidylinositol-4,5- bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice (Voronov SV et al., 2008). |
| Research Area | Neuroscience antibody |
| Calculated MW | 173 kDa |
Images (1) Click the Picture to Zoom In
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ARG52434 anti-Synaptojanin 1 antibody [5H1] WB image
Western Blot: rat hippocampal lysate showing specific immunolabeling of the ~145k synaptojanin I protein stained with Synaptojanin 1 antibody [5H1] (ARG52434).
