ARG51263
anti-Synaptotagmin antibody
anti-Synaptotagmin antibody for ICC/IF,Western blot and Human,Mouse,Rat
Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Synaptotagmin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Synaptotagmin |
| Antigen Species | Human |
| Immunogen | Peptide sequence around aa.307~311 (G-L-S-D-P) derived from Human Synaptotagmin 1. |
| Conjugation | Un-conjugated |
| Alternate Names | P65; Synaptotagmin I; SytI; SVP65; SYT; p65; Synaptotagmin-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SYT1 |
| Gene Full Name | synaptotagmin I |
| Background | The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse |
| Function | May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. [UniProt] |
| Research Area | Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 48 kDa |
Images (2) Click the Picture to Zoom In
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ARG51263 anti-Synaptotagmin antibody WB image
Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG51263 anti-Synaptotagmin antibody at 1:500 dilution.
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ARG51263 anti-Synaptotagmin antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-Synaptotagmin antibody ARG51263.
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Review for anti-Synaptotagmin antibody
Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
