ARG66881
anti-TFE3 antibody [SQab21248]
anti-TFE3 antibody [SQab21248] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab21248] recognizes TFE3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab21248 |
| Isotype | IgG |
| Target Name | TFE3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human TFE3. |
| Conjugation | Un-conjugated |
| Alternate Names | bHLHe33; TFEA; Class E basic helix-loop-helix protein 33; Transcription factor E3; RCCX1; RCCP2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TFE3 |
| Gene Full Name | transcription factor binding to IGHM enhancer 3 |
| Function | Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') (By similarity). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (By similarity). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer (PubMed:2338243). It also binds very well to a USF/MLTF site (PubMed:2338243). May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 62 kDa |
| PTM | Sumoylated; does not affect dimerization with MITF. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66881 anti-TFE3 antibody [SQab21248] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human renal cell carcinoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66881 anti-TFE3 antibody [SQab21248] for 30 min at RT.
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ARG66881 anti-TFE3 antibody [SQab21248] WB image (Customer review)
Western blot: 293T and Hela stained with ARG66881 anti-TFE3 antibody [SQab21248] at 1:500 dilution.
Customer's Feedback
Excellent
Review for anti-TFE3 antibody [SQab21248]
Application：WB
Sample：293T and HeLa
Sample Loading Amount：10 μL
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
