ARG54637
anti-TFIIB antibody [IIB8]
anti-TFIIB antibody [IIB8] for ELISA,Electrophoretic mobility shift assay,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [IIB8] recognizes TFIIB |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, EMSA, WB |
| Specificity | This antibody recognizes an epitopewithin amino acids 52-105 of humanand mouse TFIIB. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | IIB8 |
| Isotype | IgG2a |
| Target Name | TFIIB |
| Antigen Species | Human |
| Immunogen | Partially purified recombinanthuman transcription factor IIB ( |
| Conjugation | Un-conjugated |
| Alternate Names | Transcription initiation factor IIB; General transcription factor TFIIB; TF2B; TFIIB; S300-II |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity chromatography |
| Buffer | PBS (pH 7.4) |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P62915 Mouse Transcription initiation factor IIB Swiss-port # Q00403 Human Transcription initiation factor IIB |
|---|---|
| Gene Symbol | GTF2B |
| Gene Full Name | general transcription factor IIB |
| Background | This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008] |
| Function | General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 35 kDa |
Clone References
Protection against oxidative stress through SUA7/TFIIB regulation in Saccharomyces cerevisiae.
WB / Saccharomyces cerevisiae
Purification of recombinant human transcription factor IIB by immunoaffinity chromatography.
Accessibility of epitopes on human transcription factor IIB in the native protein and in a complex with DNA.
