ARG43040

anti-TRAM1 antibody

anti-TRAM1 antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TRAM1
Tested Reactivity Hu, Rat
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TRAM1
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 314-331 of Human TRAM1. (KFINFQLRRWREHSAFQA)
Conjugation Un-conjugated
Alternate Names TRAM; TRAMP; PNAS8; Translocating chain-associated membrane protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
IHC-Fr1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Thimerosal and 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23471 Human TRAM1

GeneID: 312903 Rat TRAM1

Swiss-port # Q15629 Human Translocating chain-associated membrane protein 1

Swiss-port # Q5XI41 Rat Translocating chain-associated membrane protein 1

Gene Symbol TRAM1
Gene Full Name translocation associated membrane protein 1
Background This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. [provided by RefSeq, Oct 2009]
Function Stimulatory or required for the translocation of secretory proteins across the ER membrane. [UniProt]
Cellular Localization Endoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt]
Calculated MW 43 kDa

Images (6) Click the Picture to Zoom In

  • ARG43040 anti-TRAM1 antibody ICC image

    Immunocytochemistry: HeLa cells stained with ARG43040 anti-TRAM1 antibody.

  • ARG43040 anti-TRAM1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG43040 anti-TRAM1 antibody.

  • ARG43040 anti-TRAM1 antibody WB image

    Western blot: Rat brain, Rat kidney, 293T, Raji, Jurkat and HeLa cell lysates stained with ARG43040 anti-TRAM1 antibody.

  • ARG43040 anti-TRAM1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG43040 anti-TRAM1 antibody.

  • ARG43040 anti-TRAM1 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43040 anti-TRAM1 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG43040 anti-TRAM1 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat brain tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43040 anti-TRAM1 antibody at 1 µg/ml dilution, overnight at 4°C.