ARG63116
anti-TRIM antibody [TRIM-04]
anti-TRIM antibody [TRIM-04] for Flow cytometry,Immunoprecipitation,Western blot and Human
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [TRIM-04] recognizes TRIM |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms |
| Tested Application | FACS, IP, WB |
| Specificity | The clone TRIM-04 reacts with T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TRIM-04 |
| Isotype | IgG2a |
| Target Name | TRIM |
| Antigen Species | Human |
| Immunogen | Recombinant intracellular domain (aa 29-186) of human TRIM. |
| Conjugation | Un-conjugated |
| Alternate Names | TRIM; TCRIM; pp29/30; T-cell receptor-associated transmembrane adapter 1; T-cell receptor-interacting molecule; HSPC062 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | FACS: Staining technique: Intracellular staining. WB: Under non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6PIZ9 Human T-cell receptor-associated transmembrane adapter 1 |
|---|---|
| Gene Symbol | TRAT1 |
| Gene Full Name | T cell receptor associated transmembrane adaptor 1 |
| Background | TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear. |
| Function | Stabilizes the TCR (T-cell antigen receptor)/CD3 complex at the surface of T-cells. [UniProt] |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 21 kDa |
| PTM | Phosphorylated on tyrosines by LCK or FYN upon TCR activation. |
Images (1) Click the Picture to Zoom In
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ARG63116 anti-TRIM antibody [TRIM-04] WB image
Western blot: Jurkat cell lysate (1% lauryl maltoside) (non-reduced sample) stained with ARG63116 anti-TRIM antibody [TRIM-04].
Clone References
Redundancy in antigen-presenting function of the HLA-DR and -DQ molecules in the multiple sclerosis-associated HLA-DR2 haplotype.
WB / Human
Developmentally regulated expression of the transmembrane adaptor protein trim in fetal and adult T cells.
The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain.
