ARG45433
anti-TSEN34 antibody
anti-TSEN34 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TSEN34 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TSEN34 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human TSEN34. |
| Conjugation | Un-conjugated |
| Alternate Names | TSEN34; TRNA Splicing Endonuclease Subunit 34; SEN34; SEN34L; LENG5; Leukocyte Receptor Cluster (LRC) Member 5; TSEN34 TRNA Splicing Endonuclease Subunit; TRNA-Splicing Endonuclease Subunit Sen34; Leukocyte Receptor Cluster Member 5; TRNA-Intron Endonuclease Sen34; TRNA Splicing Endonuclease 34 Homolog (SEN34, S. Cerevisiae); TRNA Splicing Endonuclease 34 Homolog (S. Cerevisiae); CTD-3093M3.1; EC 4.6.1.16; HsSen34; PCH2C |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 50 kDa |
Properties
| Form | Powder |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8BMZ5 Mouse tRNA-splicing endonuclease subunit Sen34 Swiss-port # Q9BSV6 Human tRNA-splicing endonuclease subunit Sen34 |
|---|---|
| Gene Symbol | TSEN34 |
| Gene Full Name | TRNA Splicing Endonuclease Subunit |
| Background | This gene encodes a catalytic subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from precursor tRNAs. The endonuclease complex is also associated with a pre-mRNA 3-prime end processing factor. A mutation in this gene results in the neurological disorder pontocerebellar hypoplasia type 2. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Oct 2009] |
| Function | Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 34 kDa |
