ARG62638
anti-TSP1 / Thrombospondin 1 antibody [A4.1]
anti-TSP1 / Thrombospondin 1 antibody [A4.1] for Flow cytometry,Functional study,ICC/IF,Western blot and Human,Bovine
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [A4.1] recognizes TSP1 / Thrombospondin 1 |
|---|---|
| Tested Reactivity | Hu, Bov |
| Tested Application | FACS, FuncSt, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | A4.1 |
| Isotype | IgM |
| Target Name | TSP1 / Thrombospondin 1 |
| Immunogen | Calcium-replete, native purified human TSP from the supernatant of thrombin activated platelets. |
| Conjugation | Un-conjugated |
| Alternate Names | TSP1; THBS; THBS-1; Thrombospondin-1; TSP-1; TSP |
Application Instructions
| Application Note | WB: 1 - 2 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites fluid by ammonium sulfate precipitation. |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | THBS1 |
| Gene Full Name | thrombospondin 1 |
| Background | The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008] |
| Function | Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 129 kDa |
Clone References
Mutations in FKBP14 cause a variant of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss.
ICC/IF / Human
Oxidative stress-induced attenuation of thrombospondin-1 expression in primary rat astrocytes.
WB / Rat
Role of thrombospondin 1 in macrophage inflammation in dysferlin myopathy.
BL / Human
Thrombospondin-1 regulates the normal prostate in vivo through angiogenesis and TGF-beta activation.
WB / Human
Glucose downregulation of PKG-I protein mediates increased thrombospondin1-dependent TGF-{beta} activity in vascular smooth muscle cells.
WB / Mouse
Angiostatic factors normally restrict islet endothelial cell proliferation and migration: implications for islet transplantation.
BL / Rat
PPARalpha deficiency in inflammatory cells suppresses tumor growth.
BL / Mouse
Antiangiogenic treatment with thrombospondin-1 enhances primary tumor radiation response and prevents growth of dormant pulmonary micrometastases after curative radiation therapy in human melanoma xenografts.
IHC / Human
