ARG66750
anti-TdT antibody [SQab20183]
anti-TdT antibody [SQab20183] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab20183] recognizes TdT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab20183 |
| Isotype | IgG |
| Target Name | TdT |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 1-100 of Human TdT. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.7.7.31; Terminal addition enzyme; Terminal transferase; DNA nucleotidylexotransferase; Terminal deoxynucleotidyltransferase; TDT |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Thymoma tissue. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DNTT |
| Gene Full Name | DNA nucleotidylexotransferase |
| Background | This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008] |
| Function | Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 59 kDa |
Images (2) Click the Picture to Zoom In
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ARG66750 anti-TdT antibody [SQab20183] WB image (Customer's Feedback)
Western blot: JurKat and Molt-4 cell lysate stained with ARG66750 anti-TdT antibody [SQab20183] at 1:1000 dilution.
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ARG66750 anti-TdT antibody [SQab20183] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human thymoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66750 anti-TdT antibody [SQab20183] at 18°C - 25°C for 30 minutes.
Customer's Feedback
Excellent
Review for anti-TdT antibody [SQab20183]
Application：WB
Sample：JurKat. Molt4
Sample Loading Amount：20 μg
SDS Page Type：Denatured
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
