ARG57100
anti-Thymidylate Synthase antibody [21S5]
anti-Thymidylate Synthase antibody [21S5] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [21S5] recognizes Thymidylate Synthase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 21S5 |
| Isotype | IgG2b, kappa |
| Target Name | Thymidylate Synthase |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-313 of Human Thymidylate Synthase |
| Conjugation | Un-conjugated |
| Alternate Names | Thymidylate synthase; TMS; TSase; TS; HST422; EC 2.1.1.45 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TYMS |
| Gene Full Name | thymidylate synthetase |
| Background | Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008] |
| Function | Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. [UniProt] |
| Calculated MW | 36 kDa |
Images (3) Click the Picture to Zoom In
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ARG57100 anti-Thymidylate Synthase antibody [21S5] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57100 anti-Thymidylate Synthase antibody [21S5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57100 anti-Thymidylate Synthase antibody [21S5] FACS image
Flow Cytometry: HeLa cell line stained with ARG57100 anti-Thymidylate Synthase antibody [21S5] at 2- 5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57100 anti-Thymidylate Synthase antibody [21S5] WB image
Western blot: 50 ng of 1) Recombinant Human TYMS protein, 40 µg of 2) HeLa, 3) Jurkat, and 4) MCF7 cell lysates stained with ARG57100 anti-Thymidylate Synthase antibody [21S5] at 1:2000.
