ARG55838

anti-Triosephosphate isomerase antibody

anti-Triosephosphate isomerase antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Triosephosphate isomerase
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Bov, Mk, Rb
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Triosephosphate isomerase
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 258-286 (C-terminus) of Human Triosephosphate isomerase.
Conjugation Un-conjugated
Alternate Names EC 5.3.1.1; HEL-S-49; TPID; TPI; TIM; Triosephosphate isomerase; Triose-phosphate isomerase

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HepG2

Properties

Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21991 Mouse TPI1

GeneID: 24849 Rat TPI1

GeneID: 7167 Human TPI1

Gene Symbol TPI1
Gene Full Name triosephosphate isomerase 1
Background This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Calculated MW 31 kDa
PTM The initiator methionine for isoform 2 is removed.

Images (3) Click the Picture to Zoom In

  • ARG55838 anti-Triosephosphate isomerase antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostate carcinoma stained with ARG55838 anti-Triosephosphate isomerase antibody.

  • ARG55838 anti-Triosephosphate isomerase antibody WB image

    Western blot: 35 µg of HepG2 cell lysate stained with ARG55838 anti-Triosephosphate isomerase antibody at 1:1000 dilution.

  • ARG55838 anti-Triosephosphate isomerase antibody FACS image

    Flow Cytometry: CEM cells stained with ARG55838 anti-Triosephosphate isomerase antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.