ARG63731
anti-Triosephosphate isomerase antibody
anti-Triosephosphate isomerase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Triosephosphate isomerase |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Triosephosphate isomerase |
| Antigen Species | Human |
| Immunogen | C-LKPEFVDIINAKQ |
| Conjugation | Un-conjugated |
| Alternate Names | EC 5.3.1.1; HEL-S-49; TPID; TPI; TIM; Triosephosphate isomerase; Triose-phosphate isomerase |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 31 kDa |
| PTM | The initiator methionine for isoform 2 is removed. |
Images (3) Click the Picture to Zoom In
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ARG63731 anti-Triosephosphate isomerase antibody WB image
Western Blot: human liver lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63731 anti-Triosephosphate isomerase antibody at 0.003 µg/ml dilution.
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ARG63731 anti-Triosephosphate isomerase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG63731 anti-Triosephosphate isomerase antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63731 anti-Triosephosphate isomerase antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Liver (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63731 anti-Triosephosphate isomerase antibody at 2 µg/ml dilution followed by HRP-staining.
