ARG62653
anti-Tropomyosin (sarcomeric) antibody [CH1]
anti-Tropomyosin (sarcomeric) antibody [CH1] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Rat
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [CH1] recognizes Tropomyosin |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | specific for human striated muscle forms of tropomyosin, including cardiac alpha tropomyosin and skeletal muscle forms of tropomyosin. Reacts with human striated muscle forms of tropomyosin. Not cross-react with smooth muscle or non-muscle isoforms of tropomyosin. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CH1 |
| Isotype | IgG1 |
| Target Name | Tropomyosin |
| Antigen Species | Chicken |
| Immunogen | Purified chicken muscle tropomyosin. |
| Conjugation | Un-conjugated |
| Alternate Names | C15orf13; Tropomyosin alpha-1 chain; TMSA; CMD1Y; HEL-S-265; HTM-alpha; CMH3; Tropomyosin-1; LVNC9; Alpha-tropomyosin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||||
| Positive Control | Human tongue, heart and chicken heart |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TPM1 |
| Gene Full Name | tropomyosin 1 (alpha) |
| Background | Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. Non-muscle forms of tropomyosin have been identified in a wide range of cell types. |
| Function | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 33 kDa |
| PTM | Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells. |
Images (1) Click the Picture to Zoom In
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ARG62653 anti-Tropomyosin (sarcomeric) antibody [CH1] WB image
Western blot: 50 µg of Rat heart, Rat skeletal muscle, Mouse heart, Mouse skeletal muscle, HeLa, U2OS, A549 and Caco-2 cell lysates stained with ARG62653 anti-Tropomyosin (sarcomeric) antibody [CH1] at 0.5 µg/ml, overnight at 4°C.
Clone References
eIF4EBP3L acts as a gatekeeper of TORC1 in activity-dependent muscle growth by specifically regulating Mef2ca translational initiation.
Normal myofibrillar development followed by progressive sarcomeric disruption with actin accumulations in a mouse Cfl2 knockout demonstrates requirement of cofilin-2 for muscle maintenance.
Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
