ARG63598
anti-UBE2L3 antibody
anti-UBE2L3 antibody for ICC/IF,Western blot and Human,Mouse,Rat,Pig
Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes UBE2L3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cow |
| Tested Application | ICC/IF, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | UBE2L3 |
| Antigen Species | Human |
| Immunogen | C-EFTKKYGEKRPVD |
| Conjugation | Un-conjugated |
| Alternate Names | UbcM4; Ubiquitin-protein ligase L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin carrier protein L3; E2-F1; EC 6.3.2.19; UBCH7; UbcH7; Ubiquitin-conjugating enzyme E2 L3; L-UBC |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P68036 Human Ubiquitin-conjugating enzyme E2 L3 Swiss-port # P68037 Mouse Ubiquitin-conjugating enzyme E2 L3 |
|---|---|
| Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 18 kDa |
| PTM | Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation. |
Images (6) Click the Picture to Zoom In
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ARG63598 anti-UBE2L3 antibody WB image
Western Blot: HeLa lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63598 anti-UBE2L3 antibody at 0.5 µg/ml dilution.
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ARG63598 anti-UBE2L3 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG63598 anti-UBE2L3 antibody (green) at 5 µg/ml dilution. DAPI (blue) for nuclear staining.
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ARG63598 anti-UBE2L3 antibody WB image
Western blot: 35 µg of Human (A), Mouse (B), Rat (C) and Pig (D) heart lysates (in RIPA buffer) stained with ARG63598 anti-UBE2L3 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
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ARG63598 anti-UBE2L3 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG63598 anti-UBE2L3 antibody (green) at 5 µg/ml dilution. DAPI (blue) for nuclear staining.
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ARG63598 anti-UBE2L3 antibody WB image
Western blot: 35 µg of Human (A), Mouse (B) and Rat (C) skeletal muscle lysates (in RIPA buffer) stained with ARG63598 anti-UBE2L3 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
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ARG63598 anti-UBE2L3 antibody WB image
Western blot: 35 µg of HEK293 (A), HepG2 (B) and Jurkat (C) cell lysates (in RIPA buffer) stained with ARG63598 anti-UBE2L3 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
