ARG62608
anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)]
anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog,Goat,Guinea pig,Pig,Rabbit,Sheep,Zebrafish
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [13C4 (I3C4)] recognizes UCHL1 / PGP9.5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dog, Goat, Gpig, Pig, Rb, Sheep, Zfsh |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 13C4 (I3C4) |
| Isotype | IgG |
| Target Name | UCHL1 / PGP9.5 |
| Antigen Species | Human |
| Immunogen | Human brain |
| Conjugation | Un-conjugated |
| Alternate Names | PGP95; UCH-L1; PGP9.5; PARK5; Ubiquitin thioesterase L1; HEL-117; Neuron cytoplasmic protein 9.5; Uch-L1; EC 6.-.-.-; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; NDGOA; EC 3.4.19.12 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified Antibody |
| Buffer | 1X PBS and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | UCHL1 |
| Gene Full Name | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| Background | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009] |
| Function | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody |
| Calculated MW | 25 kDa |
| PTM | O-glycosylated. |
Images (2) Click the Picture to Zoom In
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ARG62608 anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)] WB image
Western blot: 30 µg of Mouse brain lysate stained with ARG62608 anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)] at 1:1000 dilution.
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ARG62608 anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)] WB image
Western blot: 30 µg of Rat brain lysate stained with ARG62608 anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)] at 1:2000 dilution.
Customer's Feedback
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Review for anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)]
Application：WB
Sample：1) A549, 2) H1299 cells
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Telocytes as a Novel Structural Component in the Muscle Layers of the Goat Rumen.
IHC-P / Goat
