ARG55818
anti-USP11 / UHX1 antibody [1220CT620.193.189]
anti-USP11 / UHX1 antibody [1220CT620.193.189] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes USP11 / UHX1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1220CT620.193.189 |
| Isotype | IgG1, kappa |
| Target Name | USP11 / UHX1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 32-300 (C-terminus) of Human USP11. |
| Conjugation | Un-conjugated |
| Alternate Names | UHX1; Deubiquitinating enzyme 11; Ubiquitin thioesterase 11; Ubiquitin carboxyl-terminal hydrolase 11; Ubiquitin-specific-processing protease 11; EC 3.4.19.12 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Jurkat |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P51784 Human Ubiquitin carboxyl-terminal hydrolase 11 |
|---|---|
| Gene Symbol | USP11 |
| Gene Full Name | ubiquitin specific peptidase 11 |
| Background | Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23 [provided by RefSeq, Jul 2008] |
| Function | Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Note=Predominantly nuclear. Associates with chromatin |
| Calculated MW | 110 kDa |
Images (2) Click the Picture to Zoom In
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ARG55818 anti-USP11 / UHX1 antibody WB image
Western blot: 35 µg of Jurkat cell lysate stained with ARG55818 anti-USP11 / UHX1 antibody at 1:1000 dilution.
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ARG55818 anti-USP11 / UHX1 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG55818 anti-USP11 / UHX1 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
