Overview

Product Description	Rabbit Polyclonal antibody recognizes USP14
Tested Reactivity	Hu, Ms, Rat
Tested Application	FACS, ICC/IF, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	USP14
Antigen Species	Human
Immunogen	Synthetic peptide of Human USP14.
Conjugation	Un-conjugated
Alternate Names	Deubiquitinating enzyme 14; TGT; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14; Ubiquitin carboxyl-terminal hydrolase 14; EC 3.4.19.12

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:60 ICC/IF 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa
Observed Size	~ 57 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 59025 Mouse USP14 GeneID: 9097 Human USP14 Swiss-port # P54578 Human Ubiquitin carboxyl-terminal hydrolase 14 Swiss-port # Q9JMA1 Mouse Ubiquitin carboxyl-terminal hydrolase 14
Gene Symbol	USP14
Gene Full Name	ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Background	This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Function	Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). [UniProt]
Cellular Localization	Cytoplasm. Cell membrane; Peripheral membrane protein. [UniProt]
Calculated MW	56 kDa

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