ARG59512
anti-USP5 antibody
anti-USP5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes USP5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | USP5 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-280 of Human USP5 (NP_003472.2). |
| Conjugation | Un-conjugated |
| Alternate Names | Ubiquitin-specific-processing protease 5; Ubiquitin carboxyl-terminal hydrolase 5; Isopeptidase T; Deubiquitinating enzyme 5; ISOT; EC 3.4.19.12; Ubiquitin thioesterase 5 |
Application Instructions
| Predict Reactivity Note | Rat | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse kidney and Jurkat | ||||||||
| Observed Size | 110 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P45974 Human Ubiquitin carboxyl-terminal hydrolase 5 Swiss-port # P56399 Mouse Ubiquitin carboxyl-terminal hydrolase 5 |
|---|---|
| Gene Symbol | USP5 |
| Gene Full Name | ubiquitin specific peptidase 5 (isopeptidase T) |
| Background | Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010] |
| Function | Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. [UniProt] |
| Calculated MW | 96 kDa |
Images (1) Click the Picture to Zoom In
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ARG59512 anti-USP5 antibody WB image
Western blot: 25 µg of Mouse kidney and Jurkat cell lysates stained with ARG59512 anti-USP5 antibody at 1:1000 dilution.
