ARG56935
anti-VAMP2 antibody [3E5]
anti-VAMP2 antibody [3E5] for ICC/IF,Western blot and Human,Rat
Overview
| Product Description | Mouse Monoclonal antibody [3E5] recognizes VAMP2 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3E5 |
| Isotype | IgG1, kappa |
| Target Name | VAMP2 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-89 of Human VAMP2. |
| Conjugation | Un-conjugated |
| Alternate Names | SYB2; Synaptobrevin-2; Vesicle-associated membrane protein 2; VAMP-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P63027 Human Vesicle-associated membrane protein 2 Swiss-port # P63045 Rat Vesicle-associated membrane protein 2 |
|---|---|
| Gene Symbol | VAMP2 |
| Gene Full Name | vesicle-associated membrane protein 2 (synaptobrevin 2) |
| Background | The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008] |
| Function | Involved in the targeting and/or fusion of transport vesicles to their target membrane. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. [UniProt] |
| Calculated MW | 13 kDa |
| PTM | Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2. |
Images (4) Click the Picture to Zoom In
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ARG56935 anti-VAMP2 antibody [3E5] ICC/IF image
Immunoflorescense: U87MG cell line stained with ARG56935 anti-VAMP2 antibody [3E5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56935 anti-VAMP2 antibody [3E5] WB image
Western blot: 50 ng of 1) Recombinant Human VAMP1, 2) Recombinant Human VAMP2, and 3) Recombinant Human VAMP3 stained with ARG56935 anti-VAMP2 antibody [3E5] at 1:1000.
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ARG56935 anti-VAMP2 antibody [3E5] WB image
Western blot: 50 ng of Mouse brain tissue lysate stained with ARG56935 anti-VAMP2 antibody [3E5] at 1:1000.
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ARG56935 anti-VAMP2 antibody [3E5] WB image
Western blot: 20 µg of Rat brain tissue lysate stained with ARG56935 anti-VAMP2 antibody [3E5] at 1:2000.
