ARG41006
anti-VAMP2 antibody
anti-VAMP2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes VAMP2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Specificity | The antibody might also react to VAMP1 (75%) and VAMP3 (86%) based on sequence homology analysis. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VAMP2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 1-100 of Human VAMP2 (NP_055047.2). |
| Conjugation | Un-conjugated |
| Alternate Names | SYB2; Synaptobrevin-2; Vesicle-associated membrane protein 2; VAMP-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Rat brain | ||||||
| Observed Size | 16 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VAMP2 |
| Gene Full Name | vesicle-associated membrane protein 2 (synaptobrevin 2) |
| Background | The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008] |
| Function | Involved in the targeting and/or fusion of transport vesicles to their target membrane. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. [UniProt] |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Cell membrane. Note=Neuronal synaptic vesicles. Colocalizes with PRKCZ and WDFY2 in intracellular vesicles (PubMed:17313651). [UniProt] |
| Calculated MW | 13 kDa |
| PTM | Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG41006 anti-VAMP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat testis stained with ARG41006 anti-VAMP2 antibody at 1:100 dilution.
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ARG41006 anti-VAMP2 antibody WB image
Western blot: 25 µg of Rat brain lysate stained with ARG41006 anti-VAMP2 antibody at 1:3000 dilution.
