ARG41408
anti-Wnt10b antibody
anti-Wnt10b antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Wnt10b |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | IHC-P, WB |
| Specificity | Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Wnt10b |
| Antigen Species | Human |
| Immunogen | A 15 amino acid synthetic peptide around the center region of Human Wnt10b. |
| Conjugation | Un-conjugated |
| Alternate Names | WNT-12; Protein Wnt-12; SHFM6; Protein Wnt-10b |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Human skeletal muscle | ||||||
| Observed Size | ~ 47 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | WNT10B |
| Gene Full Name | wingless-type MMTV integration site family, member 10B |
| Background | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008] |
| Function | Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. Secreted. [UniProt] |
| Calculated MW | 43 kDa |
| PTM | Palmitoleylation is required for efficient binding to frizzled receptors. Depalmitoleylation leads to Wnt signaling pathway inhibition. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG41408 anti-Wnt10b antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue stained with ARG41408 anti-Wnt10b antibody at 2.5 µg/ml dilution.
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ARG41408 anti-Wnt10b antibody WB image
Western blot: Human skeletal muscle lysate stained with ARG41408 anti-Wnt10b antibody at 2 and 4 µg/ml dilution.
