ARG62662
anti-XPF antibody [51]
anti-XPF antibody [51] for Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [51] recognizes XPF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 51 |
| Isotype | IgG2b, kappa |
| Target Name | XPF |
| Antigen Species | Human |
| Immunogen | Recombinant full length protein (Human). |
| Conjugation | Un-conjugated |
| Alternate Names | DNA repair protein complementing XP-F cells; Xeroderma pigmentosum group F-complementing protein; FANCQ; XPF; EC 3.1.-.-; DNA repair endonuclease XPF; ERCC11; RAD1; DNA excision repair protein ERCC-4 |
Application Instructions
| Application Note | WB: use a concentration of 1 - 2 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | MCF-7 cells |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ERCC4 |
| Gene Full Name | excision repair cross-complementation group 4 |
| Background | XPF/ERCC4 is suggested to play a role in the repair of DNA double-strand breaks (DSB), homologous recombination, and gene conversion via single-strand annealing (SSA). XPF/ERCC4 is an endonuclease that incises 5-prime DNA. Defects in XPF/ERCC4 cause xeroderma pigmentosum VI (XP6) an autosomal recessive disease characterized by hypersensitivity to sunlight and a predisposition to skin cancer as well as neurological abnormalities. Defects in XPF/ERCC4 are also responsible for XFE progeroid syndrome, a syndrome characterized by dwarfism, cachexia, and microcephaly. |
| Function | Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link. [UniProt] |
| Cellular Localization | Nucleus |
| Research Area | Gene Regulation antibody |
| Calculated MW | 104 kDa |
Clone References
Sequential recruitment of the repair factors during NER: the role of XPG in initiating the resynthesis step.
BL / Human
gamma-H2AX formation in response to interstrand crosslinks requires XPF in human cells.
WB / Human
Phosphorylation of XPB helicase regulates TFIIH nucleotide excision repair activity.
WB / Human
