ARG62663
anti-XRCC1 antibody [33-2-5]
anti-XRCC1 antibody [33-2-5] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Rat,Hamster
Cancer antibody; Gene Regulation antibody; Metabolism antibody
Overview
| Product Description | Mouse Monoclonal antibody [33-2-5] recognizes XRCC1 |
|---|---|
| Tested Reactivity | Hu, Rat, Hm |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 33-2-5 |
| Isotype | IgG2b |
| Target Name | XRCC1 |
| Antigen Species | Human |
| Immunogen | Recombinant full length protein (Human). |
| Conjugation | Un-conjugated |
| Alternate Names | X-ray repair cross-complementing protein 1; DNA repair protein XRCC1; RCC |
Application Instructions
| Application Note | Flow Cyt: 1 µg for 10^6 cells. ICC/IF: 1/200. IHC-Fr: 1/50 - 1/100. IHC-P: 1/50 - 1/100. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | Testis |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purified |
| Buffer | PBS, 1% BSA and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | XRCC1 |
| Gene Full Name | X-ray repair complementing defective repair in Chinese hamster cells 1 |
| Background | The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008] |
| Function | Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. [UniProt] |
| Cellular Localization | Nucleus. |
| Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 69 kDa |
| PTM | Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF. Sumoylated. |
Clone References

