ARG66624
anti-YARS / TyrRS antibody
anti-YARS / TyrRS antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes YARS / TyrRS |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | YARS / TyrRS |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide around the C-terminal region of Human YARS / TyrRS. |
| Conjugation | Un-conjugated |
| Alternate Names | Tyrosyl-tRNA synthetase; CMTDIC; YTS; EC 6.1.1.1; Tyrosine--tRNA ligase, cytoplasmic; TyrRS; TYRRS; YRS |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 59 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P54577 Human Tyrosine--tRNA ligase, cytoplasmic |
|---|---|
| Gene Symbol | YARS |
| Gene Full Name | tyrosyl-tRNA synthetase |
| Background | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 59 kDa |
Images (2) Click the Picture to Zoom In
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ARG66624 anti-YARS / TyrRS antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon cancer tissue section. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66624 anti-YARS / TyrRS antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ARG66624 anti-YARS / TyrRS antibody WB image
Western blot: HeLa and U-87 MG whole cell lysates stained with ARG66624 anti-YARS / TyrRS antibody.
