ARG59525
anti-ZAK antibody
anti-ZAK antibody for ICC/IF,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ZAK |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ZAK |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 100-312 of Human ZAK (NP_598407.1). |
| Conjugation | Un-conjugated |
| Alternate Names | AZK; MLK-related kinase; mlklak; EC 2.7.11.25; MLK7; MLK-like mitogen-activated protein triple kinase; MLTK; Human cervical cancer suppressor gene 4 protein; HCCS-4; MLT; MRK; Mitogen-activated protein kinase kinase kinase MLT; pk; Sterile alpha motif- and leucine zipper-containing kinase AZK; Leucine zipper- and sterile alpha motif-containing kinase; Mixed lineage kinase-related kinase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse heart and Rat heart | ||||||
| Observed Size | 105 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9ESL4 Mouse Mitogen-activated protein kinase kinase kinase MLT |
|---|---|
| Gene Symbol | ZAK |
| Gene Full Name | sterile alpha motif and leucine zipper containing kinase AZK |
| Background | This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates to the nucleus upon ultraviolet B irradiation. [UniProt] |
| Calculated MW | 91 kDa |
Images (1) Click the Picture to Zoom In
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ARG59525 anti-ZAK antibody WB image
Western blot: 25 µg of Mouse heart and Rat heart lysates stained with ARG59525 anti-ZAK antibody at 1:3000 dilution.
