ARG59889
anti-ZO3 antibody
anti-ZO3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ZO3 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Predict Reactivity | Hm |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ZO3 |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide corresponding to aa. 885-905 of Mouse ZO3. (RARDVESSDDEGYDWGPATDL) |
| Conjugation | Un-conjugated |
| Alternate Names | ZO3; Tight junction protein ZO-3; Zonula occludens protein 3; Zona occludens protein 3; ZO-3; Tight junction protein 3 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was recommended. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 105 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TJP3 |
|---|---|
| Gene Full Name | tight junction protein 3 |
| Background | The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012] |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Nucleus. Note=Exhibits predominant nuclear expression in proliferating cells but is exclusively junctionally expressed after confluence is reached (PubMed:23608536). Shows an epithelial-specific tight junction localization in a TJP1/TJP2-dependent fashion (By similarity). [UniProt] |
| Calculated MW | 101 kDa |
Images (3) Click the Picture to Zoom In
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ARG59889 anti-ZO3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue stained with ARG59889 anti-ZO3 antibody.
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ARG59889 anti-ZO3 antibody WB image
Western blot: Rat brain and Rat heart lysates stained with ARG59889 anti-ZO3 antibody.
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ARG59889 anti-ZO3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG59889 anti-ZO3 antibody.
