ARG45832
anti-Zebrafish PHAP3 antibody
anti-Zebrafish PHAP3 antibody for IHC-Formalin-fixed paraffin-embedded sections and Zebrafish
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PHAP3 |
|---|---|
| Tested Reactivity | Zfsh |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PHAP3 |
| Antigen Species | Zebrafish |
| Conjugation | Un-conjugated |
| Alternate Names | ANP32E; acidic (leucine-rich) nuclear phosphoprotein 32 family, member E; LANPL; LANP-like protein; Acidic leucine-rich nuclear phosphoprotein 32 family member E; LANP-L |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | 0.02M PBS, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
GeneID: 406562 Zebrafish ANP32E Swiss-port # Q6NUW5 Zebrafish Acidic leucine-rich nuclear phosphoprotein 32 family member E |
|---|---|
| Gene Symbol | ANP32E |
| Gene Full Name | acidic (leucine-rich) nuclear phosphoprotein 32 family, member E |
| Background | The PHAP proteins (tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation. |
| Function | Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis. [UniProt] |
| Cellular Localization | Cytoplasm; Nucleus. [UniProt] |
| Calculated MW | 31 kDa |
| PTM | Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent (By similarity).. [UniProt] |
