ARG10363
anti-alpha Fetoprotein antibody [4A3]
anti-alpha Fetoprotein antibody [4A3] for ELISA and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody
Overview
| Product Description | Mouse Monoclonal antibody [4A3] recognizes alpha Fetoprotein |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4A3 |
| Isotype | IgG2a |
| Target Name | alpha Fetoprotein |
| Antigen Species | Human |
| Immunogen | purified human alpha-fetoprotein. |
| Conjugation | Un-conjugated |
| Alternate Names | Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein |
Application Instructions
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A affinity purified. |
| Buffer | PBS (pH 7.4) and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 1.0-2.0 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AFP |
| Gene Full Name | alpha-fetoprotein |
| Background | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008] |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody |
| Calculated MW | 69 kDa |
| PTM | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. |
Clone References
Three-dimensional perfusion bioreactor culture supports differentiation of human fetal liver cells.
sELISA / Human
