ARG55172
anti-alpha Tubulin antibody
anti-alpha Tubulin antibody for ELISA,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Chicken Polyclonal antibody recognizes alpha Tubulin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, WB |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Target Name | alpha Tubulin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (16 aa) within aa. 140-190 of Human alpha Tubulin. |
| Conjugation | Un-conjugated |
| Alternate Names | Tubulin alpha-3C/D chain; Alpha-tubulin 2; Tubulin alpha-2 chain; H2-ALPHA; Alpha-tubulin 3C/D |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Rat Brain Tissue Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TUBA3C |
| Gene Full Name | tubulin, alpha 3c |
| Background | Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots. |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt] |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 50 kDa |
Images (1) Click the Picture to Zoom In
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ARG55172 anti-alpha Tubulin antibody WB image
Western blot: rat brain tissue lysate stained with ARG55172 anti-alpha Tubulin antibody at (A) 0.5 and (B) 1 ug/ml dilution.
