ARG54472
anti-beta Lactamase antibody [8A5.A10]
anti-beta Lactamase antibody [8A5.A10] for ELISA and Bacteria
Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [8A5.A10] recognizes beta Lactamase |
|---|---|
| Tested Reactivity | Bacteria |
| Tested Application | ELISA |
| Specificity | This antibody recognizes TEM-1-type beta-lactamases. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 8A5.A10 |
| Isotype | IgG1 |
| Target Name | beta Lactamase |
| Antigen Species | E. coli |
| Immunogen | 5’-His-tagged E. coli 205 TEM-1 R+ beta-lactamase, accession no. P62593. Sequence: MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW |
| Conjugation | Un-conjugated |
Application Instructions
| Application Note | Western blot: use at 10 ug/ml. Predicted molecular weight ~29kDa. ELISA: use at 10 - 20 ug/ml (optimized for beta - lactamase on solid phase at 10 ug/ml). These are recommended concentrations. Enduser should determine optimal concentrations for their application. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G-purified |
| Buffer | PBS (pH 7.4) |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | The beta-lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta-lactams are structurally related through the presence of a core beta-lactam ring. Bacterial resistance to beta-lactams continues to increase, primarily due to microbial production of beta-lactamases. Beta-lactamases catalyze the hydrolysis of the beta-lactam bond which destroys anti-bacterial activity. Bacteria the produce TEM- or SHV-type beta-lactamases have point mutations in structural genes that have extended the substrate specificity of these betalactamases. As a result, many of the beta-lactamase-producing Gram-negative bacteria have become multi-drug resistant. |
|---|---|
| Research Area | Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 32 kDa |
Clone References
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