ARG54081
anti-c-Jun antibody
anti-c-Jun antibody for Western blot and Human
Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; AP-1 early response transcription factor study antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes Jun |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | c-Jun |
| Antigen Species | Human |
| Immunogen | Purified recombinant human c-JUN protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | AP1; AP-1; Transcription factor AP-1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; Activator protein 1; c-Jun |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 3 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | JUN |
| Gene Full Name | jun proto-oncogene |
| Background | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. |
| Function | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. [UniProt] |
| Cellular Localization | Nucleus. |
| Highlight | Related Antibody Duos and Panels: ARG30265 AP-1 early response transcription factor Antibody Duo (c-Jun, c-Fos) Related products: c-Jun antibodies; c-Jun Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; AP-1 early response transcription factor study antibody |
| Calculated MW | 36 kDa |
| PTM | Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7. Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Acetylated at Lys-271 by EP300. |
Images (2) Click the Picture to Zoom In
-
ARG54081 anti-c-Jun antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG54081 anti-c-Jun antibody at 1:1000 dilution
-
ARG54081 anti-c-Jun antibody WB image
Western blot: 30 µg of 1) HeLa, and 2) Jurkat (negative control) cell lysates stained with ARG54081 anti-c-Jun antibody at 1:1000 dilution.
Customer's Feedback
Excellent
Review for anti-c-Jun antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Average
Review for anti-c-Jun antibody
Application：WB
Sample：A498
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
